Fitness costs of various mobile genetic elements in Enterococcus faecium and Enterococcus faecalis

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Fitness costs of various mobile genetic elements in Enterococcus faecium and Enterococcus faecalis

OBJECTIVES To determine the fitness effects of various mobile genetic elements (MGEs) in Enterococcus faecium and Enterococcus faecalis when newly acquired. We also tested the hypothesis that the biological cost of vancomycin resistance plasmids could be mitigated during continuous growth in the laboratory. METHODS Different MGEs, including two conjugative transposons (CTns) of the Tn916 fami...

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Detection and Distribution of various HLAR Gene in Enterococcus faecalis and Enterococcus faecium by Multiplex-PCR

Background: In recent years, three new aminoglycoside resistance genes such as aph (3ʹ)-IIIa and ant (4ʹ)-Ia, that encode for the APH (3ʹ) and ANT (4ʹ) have also been identified. The aim of this study was to come up with a multiplex-PCR procedure for detection of aac(6ʹ)-Ie–aph(2ʹʹ)-Ia, aph(3ʹ)-IIIa, ant(4ʹ)-Ia genes in the Enterococcus spp. clinical isolates. Material and Method: 100 ...

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Characterization of vancomycin resistance in Enterococcus faecium and Enterococcus faecalis.

Vancomycin resistance in Enterococcus faecium 180, a clinical isolate from England, was studied. Resistance to vancomycin was transferable by conjugation to other enterococci. Expression of resistance was inducible and coincided with the appearance of a new membrane protein.

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VanD-type vancomycin-resistant Enterococcus faecium and Enterococcus faecalis.

Enterococcus faecium clinical isolates A902 and BM4538, which were resistant to relatively high levels of vancomycin (128 and 64 microg/ml, respectively) and to low levels of teicoplanin (4 microg/ml), and Enterococcus faecalis clinical isolates BM4539 and BM4540, which were resistant to moderate levels of vancomycin (16 microg/ml) and susceptible to teicoplanin (0.25 microg/ml), were studied. ...

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Genetic characteristics of VanA-type vancomycin-resistant Enterococcus faecalis and Enterococcus faecium in Cuba

VanA-type vancomycin-resistant enterococci isolates from bloodstream infections in Cuba were genetically characterized. Enterococcus faecalis isolates were assigned to sequence type (ST) 28, closely related to Eastern Europe, while Enterococcus faecium belonged to ST262, ST656 and ST1349, and showed different genetic profiles.

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ژورنال

عنوان ژورنال: Journal of Antimicrobial Chemotherapy

سال: 2013

ISSN: 0305-7453,1460-2091

DOI: 10.1093/jac/dkt270